Natural gene selection can produce orange corn rich in provitamin A for Africa, U.S. By Staff Reporter email@example.com | Oct 07, 2014 04:50 AM EDT Purdue researchers have identified a set of genes that can be used to naturally boost the provitamin A content of corn kernels, a finding that could help combat vitamin A deficiency in developing countries and macular degeneration in the elderly. Professor of agronomy Torbert Rocheford and fellow researchers found gene variations that can be selected to change nutritionally poor white corn into biofortified orange corn with high levels of provitamin A carotenoids - substances that the human body can convert into vitamin A. Vitamin A plays key roles in eye health and the immune system, as well as in the synthesis of certain hormones. "This study gives us the genetic blueprint to quickly and cost-effectively convert white or yellow corn to orange corn that is rich in carotenoids - and we can do so using natural plant breeding methods, not transgenics," said Rocheford, the Patterson Endowed Chair of Translational Genomics for Crop Improvement. Watch video Vitamin A deficiency causes blindness in 250,000 to 500,000 children every year, half of whom die within a year of losing their eyesight, according to the World Health Organization. The problem most severely affects children in Sub-Saharan Africa, an area in which white corn, which has minimal amounts of provitamin A carotenoids, is a dietary mainstay. Insufficient carotenoids may also contribute to macular degeneration in the elderly, a leading cause of blindness in older populations in Europe and the U.S. Previous research by Rocheford and his colleagues identified two genes that contribute to provitamin A carotenoid levels in corn kernels, but "we wanted more cookies in the jar for breeders to pick from," he said. The researchers used a combination of statistical analysis and prediction models to identify and assess the potential usefulness of genes associated with carotenoid levels in corn. They evaluated data sets from about 200 genetically diverse lines of corn at varying scopes of investigation - from the entire corn genome to stretches of DNA surrounding small sets of genes. They uncovered four genes that had not previously been linked to carotenoid levels in corn kernels. Though many genes likely contribute to carotenoid levels in corn, "we're pretty confident that our previous and current research has now identified several genes that are the major players," Rocheford said. Their study found that a combination of visually selecting corn with darker orange kernels and using a number of these favorable genes could be an effective way to rapidly convert white and yellow corn varieties to orange corn with higher levels of provitamin A and total carotenoids. "We now have the genetic information needed to begin developing a major public-private sector collaboration with the goal of providing orange corn with high levels of provitamin A to farmers throughout Sub-Saharan Africa," he said. The study also showed that using a more targeted approach to predicting the usefulness of a small set of genes was as effective as evaluating the whole corn genome, said Brenda Owens, doctoral candidate and first author of the study. "Having this smaller list of genes to select for means that we can make the improvement of carotenoid levels in corn a simpler, faster process for plant breeders," she said. Their research - in collaboration with HarvestPlus and the International Maize and Wheat Improvement Center, also known as CIMMYT - has yielded varieties of orange corn with markedly higher amounts of provitamin A carotenoids. But further efforts to produce even higher levels will be necessary to offset degradation of nutrients after harvest and reduce the amount of corn African consumers would need to eat to attain enough provitamin A, Rocheford said. Varieties of orange corn are currently being grown in Zambia, Zimbabwe, Nigeria and Ghana. An open-pollinated variety of orange corn could be available for organic and local grower operations in the U.S. by 2016, he said. The paper was published online in Genetics and is available at http://www.genetics.org/content/early/2014/09/25/genetics.114.169979.full.pdf+html A video presentation of Rocheford discussing the research behind biofortified orange corn and its implications is available athttp://docs.lib.purdue.edu/dawnordoom/2014/presentations/11/ Funding for the research was provided by the National Science Foundation; HarvestPlus; Purdue University startup and Patterson Chair funds; the U.S. Department of Agriculture-Agricultural Research Service; Cornell University startup funds; a U.S. Department of Agriculture National Needs Fellowship; and a Borlaug Fellowship. Writer: Natalie van Hoose, 765-496-2050, firstname.lastname@example.org Sources: Torbert Rocheford, 765-494-9243, email@example.com Brenda Owens, 765-426-1078, firstname.lastname@example.org ABSTRACT A foundation for provitamin A biofortification of maize: genome-wide association and genomic prediction models of carotenoid levels Brenda F. Owens 1; Alexander E. Lipka 2; Maria Magallanes-Lundback 3; Tyler Tiede 1; Christine H. Diepenbrock 4; Catherine B. Kandianis 3, 4; Eunha Kim 3; Jason Cepela 5; Maria Mateos-Hernandez 1; C. Robin Buell 1; Edward S. Buckler 2, 4, 6; Dean DellaPenna 3; Michael A. Gore 4; Torbert Rocheford 1 1 Purdue University, Department of Agronomy, West Lafayette, IN 47907 2 Cornell University, Institute for Genomic Diversity, Ithaca, NY 14853 3 Michigan State University, Department of Biochemistry and Molecular Biology, East Lansing, MI 48824 4 Cornell University, Department of Plant Breeding and Genetics, Ithaca, NY 14853 5 Michigan State University, Department of Plant Biology, East Lansing, MI 48824 6 United States Department of Agriculture-Agricultural Research Service (ARS), Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853 E-mail: email@example.com; firstname.lastname@example.org Efforts are underway for development of crops with improved levels of provitamin A carotenoids to help combat dietary vitamin A deficiency. As a global staple crop with considerable variation in kernel carotenoid composition, maize (Zea mays L.) could have widespread impact. We performed a genome-wide association study (GWAS) of quantified seed carotenoids across a panel of maize inbreds ranging from light yellow to dark orange in grain color to identify some of the key genes controlling maize grain carotenoid composition. Significant associations at the genome-wide level were detected within the coding regions of zep1 and lut1, carotenoid biosynthetic genes not previously shown to impact grain carotenoid composition in association studies, as well as within previously associated lcyE and crtRB1 genes. We leveraged existing biochemical and genomic information to identify 58 a priori candidate genes relevant to the biosynthesis and retention of carotenoids in maize to test in a pathway-level analysis. This revealed dxs2 and lut5, genes not previously associated with kernel carotenoids. In genomic prediction models, use of markers that targeted a small set of quantitative trait loci (QTL) associated with carotenoid levels in prior linkage studies were as effective as genome-wide markers for predicting carotenoid traits. Based on our GWAS, pathway-level analysis, and genomic prediction studies, we outline a flexible strategy involving use of a small number of genes that can be selected for rapid conversion of elite white grain germplasm, with minimal amounts of carotenoids, to orange grain versions containing high levels of provitamin A.